REAL TIME PCR SERVICE
Real-Time Polymerase Chain Reaction (qPCR) is a highly sensitive and quantitative molecular technique used to detect, quantify, and analyze nucleic acids in real time. It plays a vital role in gene expression studies, diagnostics, pathogen quantification, and genotyping. At IGB, we deliver reliable, precise, and fully customizable qPCR-based services tailored to your academic, clinical, or industrial research needs.
- Gene Expression Analysis - Accurate quantification of mRNA levels
- Pathogen Quantification - Determination of bacterial, viral, fungal, or parasitic loads
- Copy Number Variation (CNV) Analysis - Detection of gene duplications or deletions
- SNP Detection and Genotyping - High-resolution screening of genetic mutations
- Microbial Load Testing - Measurement of microbial presence in clinical or environmental samples
- Validation of Knockdown/Knockout Efficiency - For siRNA, shRNA, or CRISPR applications
- miRNA Quantification - Profiling of small RNA expression
- Drug Response Studies - Assessing gene modulation post-treatment
- Sample Submission - RNA, DNA, cDNA, or other biological specimens
- Nucleic Acid Extraction - Isolation of high-quality, inhibitor-free RNA/DNA
- cDNA Synthesis (if RNA) - Reverse transcription using high-efficiency enzymes
- Primer/Probe Design or Validation - Target-specific TaqMan or SYBR Green-compatible or client-supplied primers/probes
- qPCR Assay Setup - Optimized reaction conditions and thermal cycling
- Quantitative Analysis - Absolute or relative quantification via standard or comparative Ct method
- Data Interpretation - Amplification plots, melt curves, and Ct value analysis
- Amplification plots and melt curves (for SYBR Green assays)
- Ct value tables and expression data
- Comprehensive qPCR report including reagents, conditions, standard curves (if applicable), and interpretation
- Optional: Amplicon sequence confirmation upon request
- High sensitivity and quantitative precision
- Real time monitoring of target amplification
- Minimal sample volume required
- Rapid Turnaround Time: 5 to 7 working days
- Custom assays for any gene or organism of interest
- Reliable Technical Support from Sample Preparation to Data Analysis
ImmuGenix Biosciences Private Limited,
#16/2, Nattal Garden, 1st Street,
Perambur, Chennai, – 600 011
Tamil Nadu, India.
