IGB-MarabanX Plasmid Extraction Kit
The IGB-MarabanX Plasmid Extraction Kit from Immugenix Biosciences is your go-to solution for fast, reliable, and high-quality plasmid DNA purification from bacterial cultures. Designed with researchers in mind, this kit simplifies the extraction process while delivering pure, transfection-grade plasmid DNA suitable for a wide range of molecular biology applications, including PCR, sequencing, cloning, and transfection. With an optimized spin-column format and user-friendly protocol, the IGB-MarabanX kit ensures consistent results, saving you time and effort in the lab.
IGB-MarabanX kit combines cutting-edge technology with affordability and ease of use. Here’s why it stands out:
- High Purity:Achieve clean plasmid DNA free from genomic DNA, RNA, proteins, and endotoxins,
- Fast and Efficient: Complete the entire purification process in under 30 minutes, allowing you to focus on your research rather than lengthy protocols.
- High Yield: Extract up to 20 µg of plasmid DNA from 1–5 ml of E. coli culture for high-copy plasmids, and 3–10 µg for low-copy plasmids, ensuring ample DNA for your experiments.
- User-Friendly: Simple, step-by-step instructions make the kit accessible to both novice and experienced researchers, with no need for hazardous chemicals like phenol or chloroform.
- Versatile:
Compatible with a variety of bacterial strains and culture media, and suitable for both high- and low-copy plasmids. - Cost-Effective:
Offers premium performance at a competitive price.
- Cell Lysis: Bacterial cells are lysed using an optimized alkaline lysis buffer system, which selectively denatures chromosomal DNA while keeping plasmid DNA intact. RNase A is included to remove RNA contaminants, ensuring high-purity DNA.
- DNA Binding: The lysate is loaded onto a silica-membrane spin column, where plasmid DNA selectively binds in the presence of chaotropic salts, while impurities like proteins and genomic DNA are washed away.
- Elution: Pure plasmid DNA is eluted in a low-salt buffer or water (pH 7.0–8.5), ready for immediate use in downstream applications.
- This streamlined workflow eliminates the need for time-consuming ethanol precipitation or toxic organic extractions.
- PCR and Real-Time PCR: Amplify your target sequences with confidence using pure plasmid DNA.
- Sanger Sequencing: Obtain clear, reliable sequencing results with minimal background noise.
- Cloning and Gene Synthesis: Use purified plasmid DNA for restriction digestion, ligation, and transformation.
- Transfection and Transformation: Achieve high transfection efficiency in bacterial and mammalian cells with transfection-grade DNA.
- In Vitro Transcription: Generate RNA templates for functional studies.
- Restriction Enzyme Digestion: Ensure complete digestion with contaminant-free DNA.
| Product Name | Catalogue No | Pack Size |
|---|---|---|
|
IGB MarabanX Plasmid DNA Extraction Kit |
IGB-DEX-006 |
50 reaction
100 reaction
250 reaction |
- Spin Columns
- Buffers:
Buffer P1 (Resuspension Buffer with RNase A)
Buffer P2 (Lysis Buffer)Buffer P3 (Neutralization Buffer)
Buffer PW (Wash Buffer)
Buffer EB (Elution Buffer)
Detailed User ManualNote:
Before use, add the provided RNase A to Buffer P1 and store at 4°C for up to 3 months. Add ethanol to Buffer PW to achieve an 80% v/v concentration, as recommended in protocols.
Technical Specifications
Sample Volume:
1–5 ml of overnight E. coli culture (A600 > 2 U/ml).
- Plasmid Size: Suitable for plasmids from 40 bp to 40 kb.
- Yield:20–30 µg for high-copy plasmids; 3–10 µg for low-copy plasmids.
- Purity: A260/A280 ratio of 1.7–1.9; A260/A230 ratio > 2.0.
- Processing Time:60 minutes per prep.
- Storage:Stable at room temperature for up to 12 months; Buffer P1 with RNase A should be stored at 4°C after preparation.
For low-copy plasmids, yields can be enhanced by adding chloramphenicol (170 mg/L) to the culture medium to amplify copy numbers.
Immugenix Biosciences, based in Chennai, India, has been a leader in molecular diagnostics since 2018. Under the MarabanX brand, we are dedicated to delivering innovative, high-quality tools that simplify and accelerate scientific research. Our IGB-MarabanX Plasmid Extraction Kit reflects our commitment to excellence, offering performance comparable to top-tier kits from companies like GenScript and Bio-Rad, but tailored to meet the needs of budget-conscious labs.
Every IGB-MarabanX Plasmid Extraction Kit is rigorously tested for performance and consistency. Quality control includes isolating plasmid DNA from a 4 ml overnight E. coli (DH5α) culture containing a high-copy plasmid (e.g., pBluescript). A yield of >20 µg and an A260/A280 ratio of 1.7–1.9 are guaranteed, ensuring reliability for your experiments.
Streamline your plasmid DNA purification with the IGB-MarabanX Plasmid Extraction Kit. Whether you’re cloning, sequencing, or transfecting, this kit delivers the purity, yield, and speed you need to succeed. Order now and experience the Immugenix Biosciences difference!
