IGB SITE-DIRECTED MUTAGENESIS SERVICE
Site-Directed Mutagenesis (SDM) is a precise molecular biology technique used to introduce specific nucleotide changes into a DNA sequences. It plays a crucial role in functional genomics, protein engineering, and gene expression analysis. IGB offers reliable and customizable SDM solutions tailored to your research and industrial needs – with high efficiency, precision, and turnaround.
- Gene/Plasmid Submission – Plasmid / DNA template with target gene
- Primer Design – Custom mutagenic primer design using in silico tools
- Mutagenesis PCR – Amplification with high-fidelity enzymes
- DpnI Digestion – Selective removal of methylated template DNA
- Transformation – Introduction into competent E. coli cells
- Screening of Colonies – PCR screening for successful mutants
- Plasmid Isolation – Miniprep of positive clones
- Sequence Validation – Sanger sequencing to confirm mutations
- Verified mutant plasmid DNA
- Sequence chromatogram/data confirming the mutation
- Detailed mutagenesis report (includes mutation details, primer sequences, and conditions used).
- Precision editing of any desired base or region
- Customizable protocols for complex or multiple mutations
- High-Fidelity Enzymes ensure minimal off-target changes
- Fast Turnaround Time - typically 30 to 45 working days
ImmuGenix Biosciences Private Limited,
#16/2, Nattal Garden, 1st Street,
Perambur, Chennai, – 600 011
Tamil Nadu, India.
